Utilization of hardened Phaseolus lunatus Beans as a Source of Naturally Occurring Bioactive Peptides

Renata B. Araújo; Ladyslene Paula; Karla  Batista; Ailton Lemes; Thiago Oliveira; Hiasmin Neri; Elize Leonice da Rocha Santos; Paulo Ghedini; Kátia Fernandes

doi:10.21664/2238-8869.2025v14i4.8263

Authors

Renata B. Araújo Universidade Federal de Goiás https://orcid.org/0000-0003-0349-4686
Ladyslene Paula Universidade Federal de Goiás https://orcid.org/0000-0001-6265-1237
Karla Batista Universidade Federal de Goiás https://orcid.org/0000-0003-4396-032X
Ailton Lemes Universidade Federal do Rio de Janeiro https://orcid.org/0000-0001-6784-0053
Thiago Oliveira Universidade Federal de Goiás https://orcid.org/0000-0002-2825-5959
Hiasmin Neri Universidade Federal de Goiás https://orcid.org/0000-0002-0493-181X
Elize Leonice da Rocha Santos Universidade Evangélica de Goiás - UniEVANGÉLICA
Paulo Ghedini Universidade Federal de Goiás https://orcid.org/0000-0003-2104-4064
Kátia Fernandes Universidade Evangélica de Goiás - UniEVANGÉLICA https://orcid.org/0000-0002-6558-5447

DOI:

https://doi.org/10.21664/2238-8869.2025v14i4.8263

Keywords:

antioxidant, antiperoxidative, thermal-resistant, vasoactive, ACE-inhibitor

Abstract

Hardened beans are rejected by consumers and frequently used as animal feed despite their unchanged nutritional content after hardening. Particularly, hardened beans are still a rich source of protein and carbohydrate in a hungry world. Several studies have described encrypted bioactive peptides; however, little is known about naturally occurring peptides and their functionalities. In this study, naturally occurring peptides from hardened Phaseolus lunatus were extracted, partially purified, and examined regarding their biological potential. Extractor solutions were tested to obtain the maximum yield and antioxidant activity. The most nonpolar was the most effective for securing antioxidant peptides (DPPH - 962±29 and FRAP - 2567±83 µmol Trolox/mg protein). Treatment for 30min/90 ºC increased 2.6-fold (2497 µmol Trolox/mg protein) the antioxidant activity by DPPH and 1.2-fold (3149 µmol Trolox/mg protein) the FRAP assay. Antioxidant peptides in the <3kDa-fraction resisted gastric/intestinal digestion and presented anti-peroxidative activity close to the BHT. Finally, peptides presented vasorelaxant activity in the range of 30% (F<3 kDa) and 17% (hydrolyzed F<3 kDa) and ACE-inhibitory activity in the range of 15% (F<3 kDa) and 85% (hydrolyzed F<3 kDa).

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